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PH and Cardiac FunctionSummary : As a pump , the function of the heart is to fill with blood and contract , thus generating pressure to promotethe flow of blood to tissues of the body . It has been widely reported that decreasing intracellular PH , which may occurduring myocardial ischemia , impairs the ability of the heart to generate pressure by altering protein structure andfunction . Given , however , that most of these investigations have utilized skinned muscle preparations , we chose insteadto use a Langendorff isolated heart preparation ( Figure 1 ) to explore these effects at the level of the heart . Methods : 12hearts were obtained from anesthetized rats , placed on a Langendorff perfusion apparatus and perfused with a modifiedKHB solution ( similar in composition to blood ) . Left ventricular pressure was recorded using a pressure transducer*following 10 – 15 minutes of equilibration under 2 conditions : PH1 7 . 4 and PH1 6 .8 . A summary of the results obtained fromthis investigation is found in Figure 2 .200180160141Left VentricularDeveloped Pressure ( mmile). . . ( The19 8 8 8 9 90Figure 1 : Langendorff Perfusion Apparatus7. 46.8PHFigure 2: LV Developed Pressure at a PH of 7 . 4 and 6 .8
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