The work has not been graded but I like the output that was submitted to me. Is it possible for the same prof to do the next assignment I will be submitting? If possible, I will greatly appreciate it.
Consider issues related to a high throughput screen in 384-well plates to find inhibitors of the enzyme Factor XIIa.
A) Identify a suita ble fluorogenic peptide for this assay.
B) Assuming Km=18 uM and kcat= 0.53 s^-1 under the buffer conditions of the assay, calculate the time needed for 50% conversion of substrate if reactions are run at:
[S(t=0)] = 1.8uM
[E(t=0)] = 7nM
C) Calculate the time needed for 50% conversion of substrate if a competitive inhibitor (K1= 1uM) is used at a concentration of 5uM
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