please provide answer and let me know if you need the agarose gel information — it should be on the internet
3) Use the following agarose gel and information on page 44 of your lab manual to answer the questions below.I Lane 1+12: 1 kb DNA ladder Lanes 2-9: PCR products (5 uL each)Lane 10: 168 negative control Lane 11: rpoB negative control a) Lane 2 contains the (circle): l6S / rpoB PCR fragment. The molecules in this band are bp long.The mass of DNA in this band is ng and the DNA concentration of the PCR that was loaded intolane 2 was ng/ul. To set up a DNA sequencing reaction with 75 ng DNA, you need to add ul of your PCR, 1 ul of primer and ddeO to a total volume of 13 ul. b) Lane 3 contains the (circle): l6S / rpoB PCR fragment. The molecules in this band are bp long.The mass of DNA in this band is ng and the DNA concentration of the PCR that was loaded intolane 3 was ng/ul. To set up a DNA sequencing reaction with 75 ng DNA, you need to add ul of your PCR, 1 u] of primer and ddeO to a total volume of 13 ul.
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